Dopamine Receptor d1 antibody (Middle Region)

Catalog No. ABIN3044442

This anti-Dopamine Receptor d1 antibody is a Rabbit Polyclonal antibody detecting Dopamine Receptor d1 in WB and IHC. Suitable for Human, Rat and Mouse.

Quick Overview for Dopamine Receptor d1 antibody (Middle Region) (ABIN3044442)

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dopamine Receptor d1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Dopamine Receptor d1 Antibody

Binding Specificity: AA 221-242, Middle Region

Purpose: Anti-Dopamine Receptor D1/DRD1 Antibody Picoband®

Sequence: AQKQIRRIAA LERAAVHAKN CQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Dopamine Receptor D1/DRD1 Antibody (ABIN3044442). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human DRD1, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Zhang J, Xiong B, Zhen X, Zhang A. (2009). "Dopamine D1 receptor ligands: where are we now and where are we going.". Med Res Rev. 29 (2): 272-294. 2. Sunahara, R. K., Niznik, H. B., Weiner, D. M., Stormann, T. M., Brann, M. R., Kennedy, J. L., Gelernter, J. E., Rozmahel, R., Yang, Y., Israel, Y., Seeman, P., O'Dowd, B. F. :Human dopamine D1 receptor encoded by an intronless gene on chromosome 5. Nature 347: 80-83, 1990. 3. Xu, M., Moratalla, R., Gold, L. H., Hiroi, N., Koob, G. F., Graybiel, A. M., Tonegawa, S. : Dopamine D1 receptor mutant mice are deficient in striatal expression of dynorphin and in dopamine-mediated behavioral responses. Cell 79: 729-742, 1994.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Dopamine Receptor d1

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Alternative Name: DRD1

Background:

Synonyms: D (1A) dopamine receptor,Dopamine D1 receptor,DRD1,

Tissue Specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. .

Background: Dopamine receptor D1, also known as DRD1, is a human gene. It is the most highly expressed DA receptor subtype among the DA receptor family. Receptors for dopamine have been classified into two functional types, D1 and D2. They belong to the family of receptors acting through G (or guanine nucleotide-binding) proteins. D2 receptors inhibit adenylyl cyclase, but D1 receptors stimulate adenylyl cyclase and activate cyclic AMP-dependent protein kinases. Dopamine D1 and D2 receptors are targets of drug therapy in many psychomotor disorders, including Parkinson's disease and schizophrenia, and may also have a role in drug addiction and alcoholism. D1 receptors regulate neuron growth and differentiation, influence behaviour and modify dopamine D2 receptor-mediated events. And the presence of a D1 receptor gene restriction fragment length polymorphism will be helpful for future disease linkage studies. DRD1 also regulates the neurochemical architecture of the striatum and is critical for the normal expression of motor activity.

Molecular Weight: 130 kDa

UniProt: P21728

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Protein targeting to Nucleus, Feeding Behaviour, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity