GABRA1 antibody (C-Term)

Catalog No. ABIN3044445

Cited in 1 publication. The Rabbit Polyclonal anti-GABRA1 antibody (ABIN3044445) specifically detects GABRA1 in WB and IHC. The antibody is reactive with Rat, Human and Mouse samples.

Quick Overview for GABRA1 antibody (C-Term) (ABIN3044445)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GABRA1 Antibody

Binding Specificity: C-Term

Purpose: Anti-GABA A Receptor alpha 1/GABRA1 Antibody

Sequence: ATYLNREPQL KAPTPHQ

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-GABA A Receptor alpha 1/GABRA1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Human, MouseWestern blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-GABRA1 antibody (ABIN3044445)

Bao, Wang, Wei, Wang, Wuyun, Bo: "Role of microRNA-4458 in patients with non-small-cell lung cancer." in: Oncology letters, Vol. 12, Issue 5, pp. 3958-3966, (2016) (PubMed).

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABRA1

Background:

Background: GABRA1, Gamma-aminobutyric acid receptor subunit alpha-1, is a protein that in humans is encoded by the GABRA1 gene. The 1,055-bp GABRA1 clone contained an open reading frame and 260 nucleotides in the 5-prime noncoding region. The 351-amino acid sequence shows 97 % homology with its bovine counterpart. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.

Gene Full Name: gamma-aminobutyric acid type A receptor subunit alpha1

Sequence Similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

Molecular Weight: 52 kDa

Gene ID: 2554

UniProt: P14867