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TREM1 antibody (Triggering Receptor Expressed On Myeloid Cells 1) (AA 74-90) Primary Antibody

TREM1 Reactivity: Mouse IHC (fro), IHC (p), WB Host: Rabbit Polyclonal
Pubmed (1)
Catalog No. ABIN3044450
$280.00
Plus shipping costs $45.00
100 μg
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  • Target
    Binding Specificity
    AA 74-90
    Reactivity
    Mouse
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This TREM1 antibody is un-conjugated
    Application
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Triggering receptor expressed on myeloid cells 1(TREM1) detection. Tested with WB, IHC-P, IHC-F in Mouse.
    Sequence
    FTRPSEVHMG KFTLKHD
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Triggering receptor expressed on myeloid cells 1(TREM1) detection. Tested with WB, IHC-P, IHC-F in Mouse.
    Gene Name: triggering receptor expressed on myeloid cells 1
    Protein Name: Triggering receptor expressed on myeloid cells 1(TREM-1)
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence in the middle of mouse TREM1(74-90aa FTRPSEVHMGKFTLKHD).
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Zhou, Xiong, Huang, Tang, Yu, Lan: "Identification of Genes Associated with Smad3-dependent Renal Injury by RNA-seq-based Transcriptome Analysis." in: Scientific reports, Vol. 5, pp. 17901, 2016 (PubMed).

  • Target
    Alternative Name
    TREM1 (TREM1 Antibody Abstract)
    Background
    Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF), MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.

    Synonyms: CD354 antibody|OTTMUSP00000018206 antibody|TREM 1 antibody|TREM-1 antibody|TREM1 antibody|TREM1_HUMAN antibody|Triggering receptor expressed on monocytes 1 antibody|Triggering receptor expressed on myeloid cells 1 antibody|Triggering receptor TREM 1 antibody|Triggering receptor TREM1 antibody
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