SOX2 antibody (Middle Region)

Catalog No. ABIN3044477

Product Details anti-SOX2 Antibody

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Binding Specificity: AA 171-183, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Transcription factor SOX-2 SOX2 Antibody Picoband®

Sequence: YSMMQDQLGY PQH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Transcription factor SOX-2 SOX2 Antibody (ABIN3044477). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SOX2, different from the related mouse and rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Bass, A. J., Watanabe, H., Mermel, C. H., Yu, S., Perner, S., Verhaak, R. G., Kim, S. Y., Wardwell, L., Tamayo, P., Gat-Viks, I., Ramos, A. H., Woo, M. S., and 36 others. SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas. Nature Genet. 41: 1238-1242, 2009. 2. Masui, S., Nakatake, Y., Toyooka, Y., Shimosato, D., Yagi, R., Takahashi, K., Okochi, H., Okuda, A., Matoba, R., Sharov, A. A., Ko, M. S. H., Niwa, H. Pluripotency governed bySox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nature Cell Biol. 9: 625-635, 2007. 3. Rizzino A (2009). "Sox2 and Oct-3/4: a versatile pair of master regulators that orchestrate the self-renewal and pluripotency of embryonic stem cells". Wiley Interdiscip Rev Syst Biol Med 1 (2): 228-36.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-SOX2 antibody (ABIN3044477)

Yi, Xie, Liu, Wang, Xu, Liu, Li, Zhong, Peng: "Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 3628578, (2018) (PubMed).

Cheng, Wang, Li, Wu, Han, Li, Chen, Xie, Huang, Du, Zhou: "MicroRNA-184 inhibits cell proliferation and invasion, and specifically targets TNFAIP2 in Glioma." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 27, (2015) (PubMed).

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2 (SOX2 Products)

Background:

Synonyms: Transcription factor SOX-2,SOX2,

Background: SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential for maintaining self-renewal, or pluripotency of undifferentiated embryonic stem cells. Sox2 is a member of the Sox family of transcription factors, which have been shown to play key roles in many stages of mammalian development. This gene is mapped to 3q26.33. It is found that SOX2 can regulate OCT3/4 expression and maintains ES pluripotency through upstream transcription factors. SOX2 is identified as a lineage-survival oncogene in lung and esophageal squamous cell carcinoma. In addition to those, SOX2 has a critical role in maintenance of embryonic and neural stem cells and holds great promise in research involving induced pluripotency, an emerging and very promising field of regenerative medicine.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 36 kDa

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis