APOA1 antibody (AA 25-259)

Catalog No. ABIN3044502

Product Details anti-APOA1 Antibody

Target: APOA1 (Apolipoprotein A-I (APOA1))

Binding Specificity: AA 25-259

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Apolipoprotein A-I(APOA1) detection. Tested with WB, IHC-P in Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Apolipoprotein A-I(APOA1) detection. Tested with WB, IHC-P in Rat.
Gene Name: apolipoprotein A-I
Protein Name: Apolipoprotein A-I

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat APOA1 recombinant protein (Position: D25-A259). Rat APOA1 shares 61.7% and 68.6% amino acid (aa) sequence identity with human and mouse APOA1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1 (APOA1 Products)

Synonyms: Alp-1 antibody, Apoa-1 antibody, Brp-14 antibody, Ltw-1 antibody, Lvtw-1 antibody, Sep-1 antibody, Sep-2 antibody, Sep2 antibody, apoA-I antibody, apoa antibody, apoa1 antibody, cb49 antibody, wu:fb33f01 antibody, zgc:103718 antibody, MGC64335 antibody, MGC89745 antibody, Apo-AI antibody, ApoA-I antibody, APOA1 antibody, LOC100136573 antibody, apoa-i antibody, ApoAI antibody, apolipoprotein A1 antibody, apolipoprotein A-I antibody, apolipoprotein A-Ia antibody, apolipoprotein A-I S homeolog antibody, apolipoprotein A4 antibody, APOA1 antibody, Apoa1 antibody, apoa1a antibody, apoa1.S antibody, apoa1 antibody, LOC100136573 antibody, apoa-i antibody, apoa4 antibody

Background: Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

Synonyms: Apo AI antibody|Apo-AI antibody|ApoA I antibody|ApoA-I antibody|APOA1 antibody|APOA1/APOC3 fusion gene antibody|APOA1_HUMAN antibody| Apolipoprotein A I precursor antibody|Apolipoprotein A-I(1-242) antibody|Apolipoprotein A1 antibody|Apolipoprotein AI antibody| Apolipoprotein of high density lipoprotein antibody|ApolipoproteinAI antibody|Brp14 antibody|Ltw1 antibody|Lvtw1 antibody|MGC117399 antibody|Sep1 antibody|Sep2 antibody

Gene ID: 25081

UniProt: P04639

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism