IL-7 antibody (AA 26-154)

Catalog No. ABIN3044526

The Rabbit Polyclonal anti-IL-7 antibody (ABIN3044526) specifically detects IL-7 in WB and IHC. The antibody is reactive with Mouse and Rat samples.

Quick Overview for IL-7 antibody (AA 26-154) (ABIN3044526)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IL-7 Antibody

Binding Specificity: AA 26-154

Purpose: Anti-IL7 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IL7 Antibody (ABIN3044526). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse IL7 recombinant protein (Position: E26-I154). Mouse IL7 shares 56.4% and 87.6% amino acid (aa) sequence identity with human and rat IL7, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: Il7

Background:

Synonyms: Interleukin-7;IL-7;Il7;Il-7;

Tissue Specificity: Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417). In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417). .

Background: Interleukin 7  (IL-7) is a protein that in humans is encoded by the IL7 gene. The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. And it is found to be a cofactor for V (D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. In addition, this cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.

Molecular Weight: 20 kDa

Gene ID: 16196

UniProt: P10168

Pathways: JAK-STAT Signaling