NUCB2 antibody (AA 25-106)

Catalog No. ABIN3044531

The Rabbit Polyclonal anti-NUCB2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect NUCB2 in samples from Rat and Mouse.

Quick Overview for NUCB2 antibody (AA 25-106) (ABIN3044531)

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUCB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-NUCB2 Antibody

Binding Specificity: AA 25-106

Purpose: Anti-Nucleobindin 2/NUCB2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Nucleobindin 2/NUCB2 Antibody Picoband® (ABIN3044531). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse
1. "Entrez Gene: NUCB2 nucleobindin 2". 2. Barnikol-Watanabe S, Gross NA, Gotz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N (February 1995). "Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein". Biol Chem Hoppe Seyler 375 (8): 497-512. 3. DeAngelis, M., Drury, S., Doucet, J., Sherry, S., Buckley, L., Pelias, M., Deininger, P., Keats, B., Batzer, M. Construction of a BAC contig and isolation of candidate genes in the Acadian Usher syndrome (USH1C) critical region. (Abstract) Am. J. Hum. Genet. 61 (suppl.): A233 only, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NUCB2

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Alternative Name: Nucb2

Background:

Synonyms: Nucleobindin-2,DNA-binding protein NEFA,Prepronesfatin,Nesfatin-1,Nucb2,Nefa,

Tissue Specificity: Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons. .

Background: Nucleobindin-2?is a?calcium-binding?EF-hand protein?that in humans is encoded by the?NUCB2?gene. By exon trapping and sequence analysis,?the?NUCB2?sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.

Molecular Weight: 55 kDa

Gene ID: 53322

UniProt: P81117