RNH1 antibody (N-Term)

Catalog No. ABIN3044540

The Rabbit Polyclonal anti-RNH1 antibody has been validated for WB, IHC, FACS and ICC. It is suitable to detect RNH1 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for RNH1 antibody (N-Term) (ABIN3044540)

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-RNH1 Antibody

Binding Specificity: AA 2-37, N-Term

Purpose: Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband®

Sequence: SLDIQSLDIQ CEELSDARWA ELLPLLQQCQ VVRLDD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (ABIN3044540). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RNH1, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. "Entrez Gene: RNH1 ribonuclease/angiogenin inhibitor 1". 2. Weremowicz S, Fox EA, Morton CC, Vallee BL (1991). "The placental ribonuclease inhibitor (RNH) gene is located on chromosome subband 11p15.5.". Genomics 8(4): 717-21. 3. Zneimer SM, Crawford D, Schneider NR, Beutler B (1991). "Mapping of the human ribonuclease inhibitor gene (RNH) to chromosome 11p15 by in situ hybridization.". Genomics 8 (1): 175-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RNH1

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Alternative Name: RNH1

Background:

Synonyms: Ribonuclease inhibitor,Placental ribonuclease inhibitor,Placental RNase inhibitor,Ribonuclease/angiogenin inhibitor 1,RAI,RNH1,PRI, RNH,

Tissue Specificity: Widely expressed, high expression found in skeletal muscle. .

Background: Ribonuclease inhibitor is an enzyme that in humans is encoded by the RNH1 gene. Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular Rnases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover, the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.

Molecular Weight: 50 kDa

Gene ID: 6050

UniProt: P13489