HLA-B27 antibody (FITC)

Catalog No. ABIN304843

Product Details anti-HLA-B27 Antibody (FITC)

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-B27 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.

Purification: Ion exchange chromatography

Immunogen: Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.

Type of Immunogen: Cells

Clone: HLA-ABC-m3

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 0.2 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for HLA-B27

Target: HLA-B27 (HLA Class I Histocompatibility Antigen, B-27 alpha Chain (HLA-B27))

Alternative Name: HLA-B27 (HLA-B27 Products)

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response