His Tag antibody (FITC)

Catalog No. ABIN304882

This Mouse Monoclonal antibody specifically detects His Tag in IHC and FACS. It exhibits reactivity toward .

Quick Overview for His Tag antibody (FITC) (ABIN304882)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: AD1-1-10

Product Details anti-His Tag Antibody (FITC)

Sequence: HHHHHH

Specificity: Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Purification: Protein G purified

Immunogen: PAX6 transcription factor linked to histidine tag.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:10), IHC

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for His Tag

Target: His Tag

Target Type: Tag