Transferrin Receptor antibody (AA 3-28)

Catalog No. ABIN305731

This Mouse Monoclonal antibody specifically detects Transferrin Receptor in WB, IHC, ELISA, IF, IP, ICC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Chicken and Hamster.

Quick Overview for Transferrin Receptor antibody (AA 3-28) (ABIN305731)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human, Mouse, Rat, Chicken, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: H68-4

Product Details anti-Transferrin Receptor Antibody

Binding Specificity: AA 3-28

Brand: IHC-plus™

Specificity: Recognizes amino acids 3-28 of the human transferrin receptor (TfR) tail. It is predicted that the antibody may exhibit cross-reactivity with other species due to conserved amino acid sequences in the cytoplasmic tail. Please note that cross-reactivity is predicted and not guaranteed.

Purification: Immunoaffinity purified

Immunogen: Recombinant human transferrin receptor.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), ICC, IF (1 - 5 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / Transferrin Receptor

Background: Name/Gene ID: TFRC
Subfamily: Metallopeptidase M28B
Family: Protease

Synonyms: TFRC, CD71, TFR1, TRFR, TFR, TR, CD71 antigen, p90, T9, Transferrin receptor, Transferrin receptor protein 1

Gene ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis