TJP1 antibody (AA 463-1109)

Catalog No. ABIN305736

This anti-TJP1 antibody is a Rabbit Polyclonal antibody detecting TJP1 in WB, IHC, IP, ELISA, IHC (p) and IHC (fro). Suitable for Human, Mouse, Rat, Dog and Guinea Pig.

Quick Overview for TJP1 antibody (AA 463-1109) (ABIN305736)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TJP1 Antibody

Binding Specificity: AA 463-1109

Brand: IHC-plus™

Specificity: Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.

Purification: Immunoaffinity purified

Immunogen: A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Immunoprecipitation: 2-5 μg/reaction.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: TJP1 / ZO-1

Background: Name/Gene ID: TJP1
Family: Occludin

Synonyms: TJP1, ZO-1, Zona occludens protein 1, Zona occludens 1, Zonula occludens protein 1, Tight junction protein 1, ZO1, Tight junction protein ZO-1, Zonula occludens 1 protein

Gene ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization