CA2 antibody (HRP)

Catalog No. ABIN305738

The Rabbit Polyclonal anti-CA2 antibody is suitable to detect CA2 in samples from Human. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for CA2 antibody (HRP) (ABIN305738)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CA2 Antibody (HRP)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti peroxidase, anti rabbit serum, as well as purified and partially purified carbonic anhydrase II (human erythrocytes).

Purification: Ion exchange chromatography

Immunogen: Carbonic Anhydrase II (Human Erythrocytes).

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:6000), IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % gentamycin sulfate, 10 mg/mL BSA, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2 / Carbonic Anhydrase II

Background: Name/Gene ID: CA2

Synonyms: CA2, CA-II, CAII, CAC, Car2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Carbonic dehydratase, Carbonic anhydrase 2, Carbonic anhydrase B

Gene ID: 760

UniProt: P00918