Thrombospondin 1 antibody

Catalog No. ABIN305761

This Mouse Monoclonal antibody specifically detects Thrombospondin 1 in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Thrombospondin 1 antibody (ABIN305761)

Target: Thrombospondin 1 (THBS1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombospondin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Thrombospondin 1 Antibody

Specificity: Designed for sensitive detection of TSP by Western Blot. It shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Species cross-reactivity: human.

Purification: Protein G purified

Immunogen: Purified human TSP.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Reduced and non-reduced. 0.5-1.0 μg/mL for 2 hours at RT. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Preservative: Azide free

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: Thrombospondin 1

Pathways: Autophagy