CD34 antibody

Catalog No. ABIN305772

This anti-CD34 antibody is a Mouse Monoclonal antibody detecting CD34 in FACS, WB, IHC and IHC (p). Suitable for Human.

Quick Overview for CD34 antibody (ABIN305772)

Target: CD34

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD34 Antibody

Specificity: Recognizes a class 2 epitope of the 110kD human CD34 Molecule.

No Cross-Reactivity: Macaque

Purification: Protein A purified

Immunogen: Human KG-1a tumor cells.

Isotype: IgG3 kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Antibody does not work in IHC-P with samples from Macaque.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.5, 0.5 mg/mL gentamicin sulfate

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: Name/Gene ID: CD34

Synonyms: CD34, CD34 Molecule, CD34 antigen

Gene ID: 947

UniProt: P28906