HMOX1 antibody (AA 12-25)

Catalog No. ABIN305794

This Rabbit Polyclonal antibody specifically detects HMOX1 in WB, IHC and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Pig, Dog, Cow, Monkey, Hamster, Bat and Xenopus laevis.

Quick Overview for HMOX1 antibody (AA 12-25) (ABIN305794)

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivity: Human, Rat, Mouse, Pig, Dog, Cow, Monkey, Hamster, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMOX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HMOX1 Antibody

Binding Specificity: AA 12-25

Specificity: Recognizes human heme oxygenase-1 (HO-1), a 288 amino acid enzyme with a crucial role in heme catabolism, cleaving the heme ring to form biliverdin which is further processed to form bilirubin by a reductase enzyme. HO-1 acitivity is highest in the spleen where it is involved in the processing of senescent erythrocytes. HO-1expression can be increased during cellular stress, including heat shock. Baseline levels of expression are high in certain tissues, including spleen and liver. Enriched enzyme preparations are recommended for experimental analysis.

No Cross-Reactivity: Dog (Canine)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Pig, Opossum, Platypus, Xenopus (100%) Turkey, Chicken, Lizard (93%).

Purification: Purified IgG

Immunogen: Synthetic peptide with the amino acid sequence DLSEALKEATKEVHC corresponding to residues 12-25 of human heme oxygenase 1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Pig, Opossum, Platypus, Xenopus (100%), Turkey, Chicken, Lizard (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB (1:500 - 1:2000)

Usage: Western Blot: Heme oxygenase 1 demonstrates a single band of approximately 32kD. Immunohistology: LS-C11351 reacts with human spleen, kidney and airway biopsies, as well as pig arteries.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: HMOX1 / HO-1

Background: Name/Gene ID: HMOX1

Synonyms: HMOX1, BK286B10, HO-1, HSP32, Heat shock protein, 32-kD, Heme oxygenase (decycling) 1, Heme oxygenase 1, HO1

Gene ID: 3162

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome