BHMT antibody (AA 1-406)

Catalog No. ABIN306415

This Mouse Monoclonal antibody specifically detects BHMT in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for BHMT antibody (AA 1-406) (ABIN306415)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 3D6

Product Details anti-BHMT Antibody

Binding Specificity: AA 1-406

Purification: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography

Immunogen: Recombinant human BHMT (1-406aa) purified from E. coli

Isotype: IgG2a kappa

Application Details

Application Notes: Recommended dilution 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In Phosphate-Buffered Saline (pH7.4) with 0.1% Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT

Background: Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.

Pathways: Methionine Biosynthetic Process