BCL2L1 antibody (AA 3-14)

Catalog No. ABIN306551

The Sheep Polyclonal anti-BCL2L1 antibody is suitable to detect BCL2L1 in samples from Human. It has been validated for WB.

Quick Overview for BCL2L1 antibody (AA 3-14) (ABIN306551)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BCL2L1 Antibody

Binding Specificity: AA 3-14

Purpose: Sheep anti Human bcl-xl

Cross-Reactivity: Dog, Human, Mouse, Rat

Characteristics: bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cell79s response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10).

Purification: Ammonium Sulfate Precipitation

Immunogen: A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence

Isotype: IgG

Application Details

Application Notes: Application: Western Blot at 1-5 μg/mL. Positive control MCF-7 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: bcl-xl

Background: Hbcl-xl, sheep anti human bcl-xl, bclxl, bcl xl, bclx, bcl2L1, BCL2L

UniProt: Q07817

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling