BrdU antibody

Catalog No. ABIN306560

The Sheep Polyclonal anti-BrdU antibody is suitable to detect BrdU. It has been validated for FACS, IHC (p), IHC (fro), WB and IP.

Quick Overview for BrdU antibody (ABIN306560)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Sheep

Clonality: Polyclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-BrdU Antibody

Purpose: Sheep anti BrdU (bromodeoxyuridine)

Characteristics: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.

Purification: Ammonium Sulfate Precipitation

Isotype: IgG

Application Details

Application Notes: Applications: Immunoprecipitation: 25-100 μg/mL, Immunohistochemistry: 25-100 μg/mL. The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 μg/mL, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100 μg/mL. Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody. Applicable in flow cytometry for the quantification of Brdu labeled cells, indication the proliferative activity of the tested cell population.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU (Bromodeoxyuridine)

Target Type: Chemical