PPP4R1 antibody (AA 38-52)

Catalog No. ABIN306661

This Sheep Polyclonal antibody specifically detects PPP4R1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PPP4R1 antibody (AA 38-52) (ABIN306661)

Target: PPP4R1 (Protein Phosphatase 4, Regulatory Subunit 1 (PPP4R1))

Reactivity: Human, Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This PPP4R1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP4R1 Antibody

Binding Specificity: AA 38-52

Purpose: Sheep anti Human Protein Phosphatase 4 R1 (X/A)

Characteristics: Protein serine/threonine phosphatase 4R1 is a protein which is associated with the catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) and may possibly regulate the activity of the enzyme. PP4C is 65 % identical to PP2AC at the amino acid level and has been placed in the type 2A family of phosphatases. PP4C has been highly conserved between species sharing 91 % amino acid identity between human and Drosophila PP4C is predominantly localized in the nucleus in rat brain and liver but is most highly expressed in testis. Additionally, PP4C was demonstrated to be an essential enzyme in the development of Drosophila embryos. The expression of PP4C was reduced to 25 % of the normal protein level in a mutant strain of Drosophila termed centrosomes minus microtubules (cmm). An interesting characteristic of the cmm phenotype is the presence of regions of cells that are unable to complete mitosis because no microtubules exist to connect chromatin and centrosomes. This phenotype implicates PP4C in the regulation of the nucleation and/or stabilization of microtubules. These data taken together indicate that PP4 has a crucial cellular function, although a physiological substrate for PP4 has not yet been identified.

Purification: Ammonium Sulfate Precipitation

Immunogen: Antibody raised against synthetic peptide corresponding to amino acids 38 to 52 of the human protein phosphatase 4R1.

Isotype: IgG

Application Details

Application Notes: Detects protein phosphatase X/A (PP4 R1) protein by Western blot (5-10 μg/mL). Detects a 125 kDa band in bovine testes lysate. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for PPP4R1

Target: PPP4R1 (Protein Phosphatase 4, Regulatory Subunit 1 (PPP4R1))

Alternative Name: Protein Phosphatase 4 R1 (X/A)

Background: Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1, Protein Phosphatase X/A'2, PP4R1

UniProt: Q8TF05, Q8K2V1, Q8VI02