PPP4C antibody

Catalog No. ABIN306665

The Rabbit Polyclonal anti-PPP4C antibody is suitable to detect PPP4C in samples from Human, Mouse, Rat and Cow. It has been validated for WB and ICC.

Quick Overview for PPP4C antibody (ABIN306665)

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP4C antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-PPP4C Antibody

Purpose: Rabbit anti Human Protein Phosphatase X/C (PP4C)

Characteristics: A protein phosphatase is a phosphatase enzyme that removes a phosphate group from the phosphorylated amino acid residue of its substrate protein. Protein phosphorylation is one of the most common forms of reversible protein posttranslational modification (PTM), with up to 30 % of all proteins being phosphorylated at any given time. Protein kinases (PKs) are the effectors of phosphorylation and catalyse the transfer of a γ-phosphate from ATP to specific amino acids on proteins. Several hundred PKs exist in mammals and are classified into distinct super-families. Proteins are phosphorylated predominantly on Ser, Thr and Tyr residues, which account for 79.3, 16.9 and 3.8 % respectively of the phosphoproteome, at least in mammals. In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation and can be grouped into three main classes based on sequence, structure and catalytic function. The largest class of PPs is the phosphoprotein phosphatase (PPP) family comprising PP1, PP2A, PP2B, PP4, PP5, PP6 and PP7, and the protein phosphatase Mg²+- or Mn²+-dependent (PPM) family, composed primarily of PP2C.

Purification: Ammonium Sulfate Precipitation

Isotype: IgG

Application Details

Application Notes: Immunoblotting 1:500 to 1:1,000. Immunocytochemistry 1:500 to 1: 1,000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for PPP4C

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Alternative Name: Protein Phosphatase X/C (PP4C)

Background: PpX/C, rabbit anti protein phosphatase 4C X/C

UniProt: P60510, P97470, Q5BJ92, A6H772