ACP1 antibody (N-Term)

Catalog No. ABIN306667

This Sheep Polyclonal antibody specifically detects ACP1 in WB and IP. It exhibits reactivity toward Human, Rat, Mouse and Cow.

Quick Overview for ACP1 antibody (N-Term) (ABIN306667)

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Reactivity: Human, Rat, Mouse, Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: This ACP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ACP1 Antibody

Binding Specificity: N-Term

Purpose: Sheep anti Human PTP (HCPTP)

Characteristics: Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18 kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinyl-phosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1)2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.

Purification: Ammonium Sulfate Precipitation

Immunogen: The human protein, HCPTPA (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.

Isotype: IgG

Application Details

Application Notes: Antbibody can be used for Western blotting and immunoprecipitation (1-10 μg/mL. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for ACP1

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Alternative Name: LMWPTP (HCPTP)

Background: LmwPTP, sheep anti human low molecular weight protein tyrosine phosphatase, Human Red Cell Protein Tyrosine phosphatase

Molecular Weight: 18kDa

UniProt: P24666, Q9D358, P41498, P11064