CAPN1 antibody

Catalog No. ABIN306692

The Mouse Monoclonal anti-CAPN1 antibody has been validated for WB, IP and EIA. It is suitable to detect CAPN1 in samples from Human.

Quick Overview for CAPN1 antibody (ABIN306692)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 28F3

Product Details anti-CAPN1 Antibody

Cross-Reactivity: Cow, Human

Characteristics: Mouse anti calpain I, II small subunit, p30,The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and μcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.

Isotype: IgG1

Application Details

Application Notes: Use at 0.5-1 μg/mL for ELISA and western blot, 1-2 μg/mL for immunoprecipitation.

Restrictions: For Research Use only

Handling

Buffer: Provided as a concentration of 1 mg/mL in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol, 3 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: Calpain I

UniProt: P17655