Kein 5 & 8 antibody

Catalog No. ABIN306823

This Mouse Monoclonal antibody specifically detects Kein 5 & 8 in IHC (fro) and WB. It exhibits reactivity toward Human.

Quick Overview for Kein 5 & 8 antibody (ABIN306823)

Target: Kein 5 & 8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Clone: C50

Product Details

Purpose: Mouse anti Human Keratin 5 & 8

Cross-Reactivity: Human

Characteristics: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Simple epithelia express cytokeratin 8 in combination with 18. Basal cells of stratified epithelia express cytokeratin 5 with cytokeratin 14. Therefore this antibody reacts with a wide range of epithelia and their carcinomas.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation of HeLa cells and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Detects Keratin 5 & 8 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 52.5 kDa band, corresponding to Keratin 8. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: Kein 5 & 8

UniProt: P05787