Kein (4,5,6,8,10,13,18) antibody

Catalog No. ABIN306826

This Mouse Monoclonal antibody specifically detects Kein (4,5,6,8,10,13,18) in IHC (fro), IHC (p) and WB. It exhibits reactivity toward Human.

Quick Overview for Kein (4,5,6,8,10,13,18) antibody (ABIN306826)

Target: Kein (4,5,6,8,10,13,18)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: C11

Product Details

Purpose: Mouse anti Human Keratin (4,5,6,8,10,13,18)

Cross-Reactivity: Human

Characteristics: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Purification: Purified by saturated ammonium sulphate precipitation

Isotype: IgG1

Application Details

Application Notes: X1260M detects keratins 4,5,6,8,10,13 & 18 by Western blot. Immunohistochemistry can be performed on frozen tissues and paraffin sections. Optimal concentration should be evaluated by serial dilutions. X1260M reacts with a variety of normal and neoplastic epithelia. Reacting with simple epithelium and both basal and superbasal layers of cornifying and non-cornifying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: Kein (4,5,6,8,10,13,18)

UniProt: P13647