Myogenin antibody

Catalog No. ABIN306849

The Mouse Monoclonal anti-Myogenin antibody (Clone F12B) (ABIN306849) specifically detects Myogenin in WB, ELISA, IF, IP, IHC (p) and EMSA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Myogenin antibody (ABIN306849)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myogenin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: F12B

Product Details anti-Myogenin Antibody

Purpose: Mouse anti Human Myogenin

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.

Purification: Protein A/G Chromatography

Isotype: IgG1

Application Details

Application Notes: This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 μg/mg), Western blotting (1 μg/mL) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: Myogenin

Background: Myogenin, Myogenic factor 4, Myf-4

UniProt: P15173, P12979, P20428

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development