FITC antibody

Catalog No. ABIN306899

The Mouse Monoclonal anti-FITC antibody is suitable to detect FITC. It has been validated for ELISA, WB and FACS.

Quick Overview for FITC antibody (ABIN306899)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Clone: F4-1

Product Details anti-FITC Antibody

Purpose: Mouse anti FITC (Fluorescein isothiocyanate)

Characteristics: The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversion of fluorescein-labeled detection into immunoenzymatic assays.

Purification: Protein A/G Chromatography

Isotype: IgG1

Application Details

Application Notes: This antibody can be used for Western blotting (1-2 μg/mL), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 μg/mL) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.95 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (Fluorescein Isothiocyanate)

Target Type: Chemical