Nitrotyrosine antibody

Catalog No. ABIN306957

The Mouse Monoclonal anti-Nitrotyrosine antibody (Clone 2A12) (ABIN306957) specifically detects Nitrotyrosine in WB.

Quick Overview for Nitrotyrosine antibody (ABIN306957)

Target: Nitrotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2A12

Product Details anti-Nitrotyrosine Antibody

Purpose: Mouse anti Nitrotyrosine

Specificity: Nitrotyrosine

Characteristics: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.

Immunogen: 3-Nitrotyrosine-KLH

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (Suggested dilution - 1:1000) and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical