Netrin 1 antibody

Catalog No. ABIN307033

This Rabbit Polyclonal antibody specifically detects Netrin 1 in WB. It exhibits reactivity toward Human.

Quick Overview for Netrin 1 antibody (ABIN307033)

Target: Netrin 1 (NTN1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Netrin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Netrin 1 Antibody

Cross-Reactivity: Cat, Cow, Human, Macaque, Mouse, Rat

Characteristics: Netrin 1, NTN1,Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis. Netrin 1s main receptor is DCC (deleted in colorectal cancer- a putative tumor suppressor gene whose expression is lost in numerous cancers). DCC belongs to the so-called family of dependence receptors. These receptors induce apoptosis when their ligand is absent, thus conferring a state of cellular dependence on Netrin 1 availability. The DCC/netrin-1 duo regulates neuron survival during nervous system development. The mechanism(s) by which Netrin 1/ DCC triggers cell death are still unknown. The localization of DCC to lipid rafts appears to be a prerequisite for its proapoptotic activity.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from the human Netrin 1 protein

Isotype: IgG

Application Details

Application Notes: Antibody can be used for Western blotting (0.5-2 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Netrin 1

Target: Netrin 1 (NTN1)

Alternative Name: Netrin 1

NCBI Accession: NP_004813

Pathways: Regulation of Cell Size