Calreticulin antibody (C-Term)

Catalog No. ABIN307159

This Sheep Polyclonal antibody specifically detects Calreticulin in WB, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for Calreticulin antibody (C-Term) (ABIN307159)

Target: Calreticulin (CALR)

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Calreticulin Antibody

Binding Specificity: C-Term

Cross-Reactivity: Dog, Human, Mouse, Rat

Characteristics: Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells.

Purification: Ammonium Sulfate Precipitation

Immunogen: Sheep were immunized with a recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.

Isotype: IgG

Application Details

Application Notes: Antibody can be used for Western blotting (1:1000-1:5000 dilution) and immunofluorescence (1:50 dilution). Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response