MBP antibody (AA 130-136)

Catalog No. ABIN307190

The Mouse Monoclonal anti-MBP antibody has been validated for WB, IHC, ELISA, IHC (p) and IHC (fro). It is suitable to detect MBP in samples from Human, Mouse, Rat, Rabbit, Sheep, Goat and Primate.

Quick Overview for MBP antibody (AA 130-136) (ABIN307190)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Human, Mouse, Rat, Rabbit, Sheep, Goat, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MBP Antibody

Binding Specificity: AA 130-136

Brand: IHC-plus™

Specificity: Recognizes residues 130-136 in human myelin basic protein (21.5kD and 18.5kD molecular forms).

Purification: Protein G purified

Immunogen: Purified human myelin basic protein.

Type of Immunogen: Purified protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (10 - 20 μg/mL), IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, contains no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein / MBP

Background: Name/Gene ID: MBP

Synonyms: MBP, Myelin A1 protein, Myelin basic protein

Gene ID: 4155

UniProt: P02686