Clathrin Heavy Chain (CLTC) antibody

Catalog No. ABIN307323

The Mouse Monoclonal anti-Clathrin Heavy Chain antibody (ABIN307323) specifically detects Clathrin Heavy Chain in WB, IHC and IHC (fro). The antibody is reactive with Human, Rat, Cow and Pig samples.

Quick Overview for Clathrin Heavy Chain (CLTC) antibody (ABIN307323)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human, Rat, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details

Specificity: Species cross-reactivity: Human, bovine, porcine, rat, amphibia. Reactivities on Cultured HeLa, SV-40, RVF-SMC Cell Lines (tested so far). Specifically Detected organs and tissues (egg brain, mammary gland, ovaries). This clone represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e. g. refs. 2 and 3). Polypeptide Reacting Mr 180000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues.

Purification: Purified

Immunogen: Coated vesicles (clathrin) of bovine brain.

Type of Immunogen: Cell extract

Isotype: IgM

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB (1:100 - 1:500)

Usage: Suitable for use in Immunohistochemistry (frozen tissue) and Western Blot (Western). Immunohistochemistry: 1:10 with PBS,  pH 7.4, Incubation Time 1 h at RT. Western Blot: 1:100-1:500 (ECL).

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin, (CLTC/CLTCL1)