PAR1 antibody (AA 27-102)

Catalog No. ABIN307496

The Goat Polyclonal anti-PAR1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect PAR1 in samples from Human.

Quick Overview for PAR1 antibody (AA 27-102) (ABIN307496)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PAR1 Antibody

Binding Specificity: AA 27-102

Specificity: Selected for its ability to recognize human PAR1 in the applications listed.

Purification: Affinity purified

Immunogen: E. coli-derived recombinant human PAR1 (aa27-102, 375-428).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody was tested in flow cytometry using whole blood granulocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 0.2 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R / Thrombin Receptor / PAR1

Background: Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

Gene ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus