GRIA4 antibody (Internal Region)

Catalog No. ABIN308410

This Goat Polyclonal antibody specifically detects GRIA4 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for GRIA4 antibody (Internal Region) (ABIN308410)

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GRIA4 Antibody

Binding Specificity: Internal Region

Purpose: GRIA4

Sequence: KKLDQREYPG SETP

Specificity: This antibody is expected to recognize all reported isforms (NP_000820.3, NP_001070711.1, NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3, NP_001070711.1, NP_001070712.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx.100 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kDa according to NP_001070711.2). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRIA4

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Alternative Name: GRIA4

Background: GRIA4 , glutamate receptor, ionotrophic, AMPA 4 , GLUR4, GLUR4C, GLURD, AMPA-selective glutamate receptor 4

Gene ID: 2893, 14802, 29629

NCBI Accession: NP_000820, NP_001070711, NP_001070712

Pathways: PI3K-Akt Signaling