ERN1 antibody (Internal Region)

Catalog No. ABIN308413

This Goat Polyclonal antibody specifically detects ERN1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for ERN1 antibody (Internal Region) (ABIN308413)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ERN1 Antibody

Binding Specificity: Internal Region

Purpose: ERN1 / IRE1a

Sequence: PNAHGKIKAM ISD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PNAHGKIKAMISD, from the internal region of the protein sequence according to NP_001424.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in Human Pancreas and Duodenum lysates (calculated MW of 110 kDa according to NP_001424.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: ERN1

Background: ERN1, IRE1, endoplasmic reticulum to nucleus signaling 1, FLJ30999, IRE1P, MGC163277, MGC163279, ER to nucleus signalling 1, endoplasmic reticulum to nucleus signalling 1, inositol-requiring 1, protein kinase/endoribonuclease, IRE1a, IRE1-alpha

Gene ID: 2081, 78943, 498013

NCBI Accession: NP_001424

Pathways: ER-Nucleus Signaling, Unfolded Protein Response