CHKB antibody (Internal Region)

Catalog No. ABIN308437

This Goat Polyclonal antibody specifically detects CHKB in ELISA. It exhibits reactivity toward Human.

Quick Overview for CHKB antibody (Internal Region) (ABIN308437)

Target: CHKB (Choline Kinase beta (CHKB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHKB antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-CHKB Antibody

Binding Specificity: Internal Region

Purpose: CHKB

Sequence: KGETLSQEEQ RKLEED

Specificity: This antibody is expected to recognize isoform a (NP_005189.2) only.

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KGETLSQEEQRKLEED, from the internal region of the protein sequence according to NP_005189.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 38 kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHKB

Target: CHKB (Choline Kinase beta (CHKB))

Alternative Name: CHKB

Background: CHKB, choline kinase beta, CHETK, CHKL, choline kinase-like, choline/ethanolamine kinase

Gene ID: 1120

NCBI Accession: NP_005189