CHKB antibody (AA 270-350)

Catalog No. ABIN7214196

This Rabbit Polyclonal antibody specifically detects CHKB in ELISA, WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CHKB antibody (AA 270-350) (ABIN7214196)

Target: CHKB (Choline Kinase beta (CHKB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHKB antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CHKB Antibody

Binding Specificity: AA 270-350

Purpose: Rabbit Anti-ChoKB Polyclonal Antibody

Specificity: ChoKB Polyclonal Antibody detects endogenous levels of ChoKB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human ChoKB at AA range: 270-350

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CHKB

Target: CHKB (Choline Kinase beta (CHKB))

Alternative Name: ChoKB

Background: CHKB, CHETK, CHKL, Choline/ethanolamine kinase, Choline kinase beta, CK, CKB, Choline kinase-like protein, Ethanolamine kinase, EK, Ethanolamine kinase beta, EKB, choline/ethanolamine kinase beta, CKEKBCholine kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family, however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus.

Molecular Weight: 45kD

Gene ID: 1120

UniProt: Q9Y259