Septin 7 antibody (Internal Region)

Catalog No. ABIN308440

This Goat Polyclonal antibody specifically detects Septin 7 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Septin 7 antibody (Internal Region) (ABIN308440)

Target: Septin 7 (SEPT7)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Septin 7 Antibody

Binding Specificity: Internal Region

Purpose: SEPT7

Sequence: DNNKNKGQLT KSP

Specificity: This antibody is expected to recognize all three reported isoforms (NP_001779.3, NP_001011553.2, NP_001229885.1).

Cross-Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DNNKNKGQLTKSP, from the internal region of the protein sequence according to NP_001779.3, NP_001011553.2, NP_001229885.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.7 kDa according to NP_001779.3 and of 55.6 kDa according to NP_001011553.2). This molecular weight is routinely observed by other sources. Recommended concen

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Septin 7

Target: Septin 7 (SEPT7)

Alternative Name: Sep-07

Background: SEPT7, septin 7, CDC10, CDC3, Nbla02942, SEPT7A, CDC10 (cell division cycle 10, S. cerevisiae, homolog), CDC10 cell division cycle 10 homolog, cell division cycle 10

Gene ID: 989, 235072, 64551

NCBI Accession: NP_001779, NP_001011553, NP_001229885