PARP3 antibody (Internal Region)

Catalog No. ABIN308468

The Goat Polyclonal anti-PARP3 antibody has been validated for ELISA and IHC. It is suitable to detect PARP3 in samples from Human.

Quick Overview for PARP3 antibody (Internal Region) (ABIN308468)

Target: PARP3 (Poly (ADP-Ribose) Polymerase Family, Member 3 (PARP3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PARP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PARP3 Antibody

Binding Specificity: Internal Region

Purpose: PARP3

Sequence: YEDYNCTLNQ TNIEN

Specificity: This antibody is expected to recognize both reported isoforms (NP_001003931.2, NP_001003935.2).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence YEDYNCTLNQTNIEN , from the internal region of the protein sequence according to NP_001003931.2, NP_001003935.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments in lysates of cell lines Daudi, HeLa and HepG2 gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PARP3

Target: PARP3 (Poly (ADP-Ribose) Polymerase Family, Member 3 (PARP3))

Alternative Name: PARP3

Background: PARP3, poly (ADP-ribose) polymerase family, member 3, ADPRT3, ADPRTL2, ADPRTL3, IRT1, hPARP-3, pADPRT-3, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 2, ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase)-like 3, NAD+ ADP-ribos

Gene ID: 10039

NCBI Accession: NP_001003931, NP_001003935