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Cytokeratin 13 antibody (C-Term)

This Goat Polyclonal antibody specifically detects Cytokeratin 13 in WB, IHC and ELISA. It exhibits reactivity toward Human.
Catalog No. ABIN308470

Quick Overview for Cytokeratin 13 antibody (C-Term) (ABIN308470)

Target

See all Cytokeratin 13 (KRT13) Antibodies
Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivity

  • 91
  • 36
  • 32
  • 11
  • 6
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 64
  • 33
  • 1
Goat

Clonality

  • 56
  • 42
Polyclonal

Conjugate

  • 55
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Cytokeratin 13 antibody is un-conjugated

Application

  • 61
  • 44
  • 17
  • 17
  • 16
  • 15
  • 13
  • 12
  • 11
  • 11
  • 7
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade

Verified
  • Binding Specificity

    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Purpose

    KRT13

    Sequence

    TSNASGRRTS DVRRP

    Specificity

    This antibody is expected to recognize the reported isoform a (NP_002265.2) only.

    Cross-Reactivity

    Human

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-TSNASGRRTSDVRRP, from the C Terminus of the protein sequence according to NP_705694.2.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Tonsil shows strong layered, filamentous staining. Recommended concentration, 2-4 μg/mL.

    Western Blot: Approx. 55 kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6 kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan,

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

    Alternative Name

    KRT13

    Background

    KRT13, keratin 13, CK13, K13, MGC161462, MGC3781, cytokeratin 13, keratin, type I cytoskeletal 13

    Gene ID

    3860

    NCBI Accession

    NP_705694
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