Cytokeratin 13 antibody (C-Term)

Catalog No. ABIN308470

This Goat Polyclonal antibody specifically detects Cytokeratin 13 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 13 antibody (C-Term) (ABIN308470)

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Cytokeratin 13 Antibody

Binding Specificity: C-Term

Purpose: KRT13

Sequence: TSNASGRRTS DVRRP

Specificity: This antibody is expected to recognize the reported isoform a (NP_002265.2) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TSNASGRRTSDVRRP, from the C Terminus of the protein sequence according to NP_705694.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows strong layered, filamentous staining. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx. 55 kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6 kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan,

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: KRT13

Background: KRT13, keratin 13, CK13, K13, MGC161462, MGC3781, cytokeratin 13, keratin, type I cytoskeletal 13

Gene ID: 3860

NCBI Accession: NP_705694