Cytokeratin 13 antibody (C-Term)
Quick Overview for Cytokeratin 13 antibody (C-Term) (ABIN308470)
Target
See all Cytokeratin 13 (KRT13) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
-
-
Binding Specificity
- C-Term
-
Purpose
- KRT13
-
Sequence
- TSNASGRRTS DVRRP
-
Specificity
- This antibody is expected to recognize the reported isoform a (NP_002265.2) only.
-
Cross-Reactivity
- Human
-
Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
Immunogen
- Peptide with sequence C-TSNASGRRTSDVRRP, from the C Terminus of the protein sequence according to NP_705694.2.
-
Isotype
- IgG
-
-
-
-
Application Notes
-
Immunohistochemistry: In paraffin embedded Human Tonsil shows strong layered, filamentous staining. Recommended concentration, 2-4 μg/mL.
Western Blot: Approx. 55 kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6 kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan,
Peptide ELISA: antibody detection limit dilution 1:128000.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 0.5 mg/mL
-
Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
- Minimize freezing and thawing.
-
Storage
- -20 °C
-
Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))
-
Alternative Name
- KRT13
-
Background
- KRT13, keratin 13, CK13, K13, MGC161462, MGC3781, cytokeratin 13, keratin, type I cytoskeletal 13
-
Gene ID
- 3860
-
NCBI Accession
- NP_705694
Target
-