GOGAT antibody

Catalog No. ABIN3155732

The Rabbit Polyclonal anti-GOGAT antibody (ABIN3155732) specifically detects GOGAT in WB. The antibody is reactive with Arabidopsis thaliana, Green bean, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Populus tremula, Spartina, Tomato and Zea mays samples.

Quick Overview for GOGAT antibody (ABIN3155732)

Target: GOGAT (Glutamine Oxoglutarate Aminotransferase (GOGAT))

Reactivity: Arabidopsis thaliana, Green bean, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Populus tremula, Spartina, Tomato, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-GOGAT Antibody

Purpose: Anti-GOGAT | Glutamine oxoglutarate aminotransferase

Cross-Reactivity (Details): Arabidopsis thaliana, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Populus tremula, Solanum lycopersicum, Spartina sp., Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Arthrospira platensis PCC 7345,Beta vulgaris,Brachypodium distachyon,Burkholderia glumae,Camellia sinensis,Chloroflexi bacterium,Chlamydonas reinhardtii,Gracilaria tenustipitata,Cyanobacteria,Crocosphaera watsonii WH 8502,Cyanidioschyzon merolae (strain 10D),Galdieria sulphuraria,Glycine max,Helicosporidium sp. ATCC 50920,Hydrogenobaculum sp. HO,Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2,Leptospira interrogans serovar Bataviae str. HAI135,Leptolyngbya boryana,Medicago trucutula,Microcystis panniformis,Monoraphidium neglectum,Morus notabilis,Nannochloropsis gaditana,Nicotiana tabacum,Ostreococcus lucimarinus,Physcomitrium patens subsp. patens,Porphyra purpurea,Sutterella wadsworthensis,Sulfurihydrogenibium yellowstonense,Veillonella atypica

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7

Application Details

Application Notes: 1 : 1000 (WB)

Comment:

A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for GOGAT

Target: GOGAT (Glutamine Oxoglutarate Aminotransferase (GOGAT))

Background: Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95 % of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.

Molecular Weight: 177 | 170-180 kDa depending upon the species