TRF1 antibody

Catalog No. ABIN317052

The Mouse Monoclonal anti-TRF1 antibody has been validated for WB, IHC (p), IHC (fro) and EIA. It is suitable to detect TRF1 in samples from Human.

Quick Overview for TRF1 antibody (ABIN317052)

Target: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: BED5

Product Details anti-TRF1 Antibody

Specificity: This antibody recognizes TTAGGG repeat binding factor 1 (TRF1) a ubiquitously expressed 60 kDa protein.

Characteristics: Synonyms: PIN2, TRBF1, TRF, TRF1, Telomeric repeat-binding factor 1, TTAGGG repeat-binding factor 1,NIMA-interacting protein 2, Telomeric protein Pin2/TRF1

Purification: Purified IgG prepared by affinity chromatography on Protein G

Immunogen: Recombinant TRF1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/200 - 1/1000, This product requires antigenretrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Western Blot: detects a band of approximately 60 kDa in Jurkat whole cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.

Expiry Date: 12 months

Target Details for TRF1

Target: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Alternative Name: TERF1

Background: Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited. Additionally in conjunction with POT1 and TTAGGG repeat binding factor 1 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1, and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage. TRF1 is thought to play a role in leukaemia being expressed at significantly higher levels in patients with acute lymphoblastic leukaemia than those with myeloid leukaemia.Synonyms: NIMA-interacting protein 2, PIN2, TRBF1, TRF, TRF1, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1, Telomeric repeat-binding factor 1

Gene ID: 7013

UniProt: P54274

Pathways: Cell Division Cycle, Telomere Maintenance, Hormone Transport, Chromatin Binding, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity