RSV antibody

Catalog No. ABIN317238

The Mouse Monoclonal anti-RSV antibody (Clone 681) (ABIN317238) specifically detects RSV in IF and EIA. The antibody is reactive with Respiratory Syncytial Virus (RSV) samples.

Quick Overview for RSV antibody (ABIN317238)

Target: RSV (Respiratory Syncytial Virus (RSV))

Reactivity: Respiratory Syncytial Virus (RSV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This RSV antibody is un-conjugated

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: 681

Product Details anti-RSV Antibody

Characteristics: Synonyms: RSV, HRSV, Fusion protein, Fusion glycoprotein F0, Protein F

Purification: Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/20-1/200. Immunofluorescence: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for RSV

Target: RSV (Respiratory Syncytial Virus (RSV))

Alternative Name: Respiratory Syncytial Virus / RSV

Target Type: Virus

Background: Respiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.