Sialoadhesin/CD169 antibody

Catalog No. ABIN317446

The Rat Monoclonal anti-Sialoadhesin/CD169 antibody (Clone 3D6-112) (ABIN317446) specifically detects Sialoadhesin/CD169 in FACS, IHC (fro) and IF. The antibody is reactive with Mouse samples.

Quick Overview for Sialoadhesin/CD169 antibody (ABIN317446)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sialoadhesin/CD169 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: 3D6-112

Product Details anti-Sialoadhesin/CD169 Antibody

Specificity: This antibody recognises Mouse CD169, a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Characteristics: Synonyms: Sialoadhesin, Siglec-1, Sialic acid-binding Ig-like lectin 1

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Purified Sialoadhesin. Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cellline.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1/50-1/100 (Fixation with either 2 % paraformaldehyde or ethanol is recommended). Flow Cytometry: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Alternative Name: CD169 / SIGLEC1

Background: Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker, myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells, CD33, a myeloid differentiation antigen, and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1

Gene ID: 20612

UniProt: Q62230