LPAR1 antibody

Catalog No. ABIN317688

The Rabbit Polyclonal anti-LPAR1 antibody (ABIN317688) specifically detects LPAR1 in WB and IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for LPAR1 antibody (ABIN317688)

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LPAR1 Antibody

Specificity: This antibody detects endogenous levels of EDG-2 protein.(region surrounding Trp45)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LPAR1

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Alternative Name: EDG-2 / LPAR1

Background: The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.Synonyms: EDG2, LPA receptor 1, LPA-1, LPA1, LPAR1, Lysophosphatidic acid receptor 1, Lysophosphatidic acid receptor Edg-2

Molecular Weight: approx. 38 kDa

Gene ID: 1902

NCBI Accession: NP_001392

UniProt: Q92633

Pathways: Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration