S1PR4 antibody

Catalog No. ABIN317690

The Rabbit Polyclonal anti-S1PR4 antibody is suitable to detect S1PR4 in samples from Human and Mouse. It has been validated for WB, IF and EIA.

Quick Overview for S1PR4 antibody (ABIN317690)

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-S1PR4 Antibody

Specificity: This antibody detects endogenous levels of EDG-6 protein.(region surrounding Ser368)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for S1PR4

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Alternative Name: S1PR4 / EDG6

Background: The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain.Synonyms: EDG-6, Endothelial differentiation G-protein coupled receptor 6, S1P receptor Edg-6, Sphingosine 1-phosphate receptor 4, Sphingosine 1-phosphate receptor Edg-6

Molecular Weight: approx. 40 kDa

Gene ID: 8698

NCBI Accession: NP_003766

UniProt: O95977