O3FAR1 antibody

Catalog No. ABIN317737

This Rabbit Polyclonal antibody specifically detects O3FAR1 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for O3FAR1 antibody (ABIN317737)

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This O3FAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-O3FAR1 Antibody

Specificity: This antibody detects endogenous levels of GPR120 protein. (region surrounding Val259)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/1000-1/10000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for O3FAR1

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Alternative Name: GPR120

Background: GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake.GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.Synonyms: G-protein coupled receptor 120, GPR129, GT01, PGR4

Molecular Weight: approx. 38 kDa

Gene ID: 338557

NCBI Accession: NP_001182684

UniProt: Q5NUL3

Pathways: Hormone Transport