MAPK6 antibody

Catalog No. ABIN317885

The Rabbit Polyclonal anti-MAPK6 antibody is suitable to detect MAPK6 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for MAPK6 antibody (ABIN317885)

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAPK6 Antibody

Specificity: ERK3 antibody detects endogenous levels of ERK3 protein.(region surrounding Lys185)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: MAPK6 / ERK3

Background: Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl2 and caspase 3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK3 gene maps to chromosome 15q21 and encodes a 721 amino acid protein.Synonyms: Extracellular signal-regulated kinase 3, MAP kinase isoform p97, Mitogen-activated protein kinase 6, Serine/threonine-protein kinase, p97-MAPK

Molecular Weight: approx. 83 kDa

Gene ID: 5597

NCBI Accession: NP_002739

UniProt: Q16659

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C