CYB5R3 antibody

Catalog No. ABIN317921

The Rabbit Polyclonal anti-CYB5R3 antibody is suitable to detect CYB5R3 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for CYB5R3 antibody (ABIN317921)

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYB5R3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CYB5R3 Antibody

Specificity: CYB5R3 antibody detects endogenous levels of CYB5R3 protein.(region surrounding Ile169)

Purification: Affinity Chromatography.

Application Details

Application Notes: ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Alternative Name: CYB5R3

Background: CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane,while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis,drug metabolism and, in erythrocytes, methemoglobin reduction. A serine residue at position 117 seems to only be found in persons of African origin. The allele frequency is 0.23 in African Americans. It is not found in Caucasians,Asians, Indo-Aryans or Arabs. This difference seems to have no effect on the enzyme activity.Synonyms: B5R, DIA1, Diaphorase-1, NADH-cytochrome b5 reductase 3

Molecular Weight: approx. 34 kDa

Gene ID: 1727

NCBI Accession: NP_000389

UniProt: P00387

Pathways: SARS-CoV-2 Protein Interactome