NFKBIA antibody (pSer32, pSer36)

Catalog No. ABIN318052

This Rabbit Polyclonal antibody specifically detects NFKBIA in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NFKBIA antibody (pSer32, pSer36) (ABIN318052)

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFKBIA Antibody

Binding Specificity: pSer32, pSer36

Specificity: This antibody detects endogenous levels of IkB-alpha pSer32/36 protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Alternative Name: NFKBIA / IKBA

Background: On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α (a 35-37 kDa protein), includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFκB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through Ankyrin repeats. The second member of the IκB family is represented by a 45 kDa protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, a 70 kDa protein identical in sequence with the C-terminal domain of the p110 precursor of NFκB p50 and expressed predominantly in lymphoid cells. An additional IκB family member has been identified as IκB-ε, a 45 kDa protein which has several phosphorylated forms and is primarily found complexed with RelA and/or c-Rel.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI

Molecular Weight: approx. 34 kDa

Gene ID: 4792

NCBI Accession: NP_065390

UniProt: P25963

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling